Diagnosis of Hemophilia and Other
Bleeding Disorders: A Laboratory Manual

This WFH manual is the definitive
how-to guide for the accurate diagnosis of hemophilia and other bleeding disorders. Written and reviewed by the world’s leading experts in coagulation testing, this comprehensive, revised edition features new sections on two-stage clotting and chromogenic FVIII assays, von Willebrand factor collagen binding assay and multimer analysis, and the use of coagulometers.

Click here to download the complete PDF, or select individual sections below.

Contents

1. Laboratory Equipment
2. Checking Pipette and Weighing Balance Calibration
3. Laboratory Safety
4. Sample Collection and Pre-Analytical Variables
5. Internal Quality Control and External Quality Assessment
6. Manual Tilt-Tube Technique
7. Preparation and Calibration of Pooled Normal Plasma (PNP)
8. Establishing a Normal Reference Range
9. Reagents
10. Platelet Count
11. Bleeding Time
12. Prothrombin Time (PT)
13. Activated Partial Thromboplastin Time (APTT)
14. Mixing Tests For Further Investigation of Abnormal PT and APTT
15. Thrombin Clotting Time
16. Thrombin Time in the Presence of Protamine Sulphate to Detect Presence of Heparin
17. Reptilase Time
18. Fibrinogen (Modifi ed Clauss Assay)
19. Removal of Heparin from Plasma
20. Fibrinogen Antigen Assay by Radial Immunodiffusion (RID)
21. Factor XIII Screen
22. Assays Based on Prothrombin Time (Factors II, V, VII, or X)
23. Factor Assays Based on APTT (One-Stage Assay of FVIII:C, FIX, FXI, or FXII)
24. Factor VIII:C Assays in Cryoprecipitate
25. Two-Stage Clotting Assay for Factor VIII:C
    25.1 Production of Combined Reagent for Two-Stage Factor FVIII:C Assay
26. Chromogenic FVIII:C Assay
27. One-Stage Intrinsic Assay of Prekallikrein (PKK) and High Molecular Weight Kininogen (HMWK)
28. Clotting Factor Inhibitor Screen Based on APTT
29. Ristocetin Cofactor Activity/von Willebrand Factor Activity (VWF: RCo or VWF:Act)
    29.1 Fixed Platelet Preparation
30. Von Willebrand Factor Antigen by Elisa (VWF:Ag)
31. Von Willebrand Factor Collagen Binding Assay (VWF:CB)
32. Factor VIII Binding Assay for Diagnosis of von Willebrand Disease Normandy
33. VWF Multimer Analysis
34. Quantitative Measurement of FVIII Inhibitors
35. Factor IX Inhibitor Assay
36. Factor XIII Activity Assay
37. Lupus Anticoagulant and Anti-Phospholipid Antibodies
38. Dilute Russell’s Viper Venom Time (DRVVT)
    38.1 Preparation of Washed Platelets for DRVVT
39. Platelet Function Testing
    39.1 Clot Retraction
    39.2 Measurement of Platelet Aggregation
40. Lyophilization of Plasma
41. Evaluation and Use of Coagulometers
42. Molecular Genetic Analysis

Last updated September 2011